Hotspot

atpF-atpH

LSC · 15571-16303 · 733 bp

Back to Pithecellobium

Pi 0.0196

MarkerSeek score 72.50

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 733
variable_sites: 14
pi: 0.0196
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	TGGGCTGGTTGTAGCATTAG	50.0	50.0	57.9	57.6	835-851 bp	1.0	78.8
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	AGGCAGAGGGAAAAATACGG	50.0	50.0	57.9	57.6	902-918 bp	1.0	78.6
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	ACGATTTATGGGCTGGTTGT	50.0	45.0	57.9	57.5	843-859 bp	1.0	78.1
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GCAGAGGGAAAAATACGGGG	50.0	55.0	57.9	58.6	900-916 bp	1.0	77.1
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	GGCAGAGGGAAAAATACGGG	50.0	55.0	57.9	58.6	901-917 bp	1.0	77.1