Hotspot

trnS-GCU-trnG-UCC

LSC · 9818-10973 · 1156 bp

Back to Pithecellobium

Pi 0.0241

MarkerSeek score 70.50

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1156
variable_sites: 26
pi: 0.0241
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	TGAATCAAACGAGGGATCCC	50.0	50.0	58.0	57.3	1212-1268 bp	1.0	80.0
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	TGAATCAAACGAGGGATCCC	50.0	50.0	58.0	57.3	1211-1267 bp	1.0	80.0
trnS-GCU-trnG-UCC_p3	ACGGAAAGAGAGGGATTCGA	TGAATCAAACGAGGGATCCC	50.0	50.0	58.2	57.3	1266-1322 bp	1.0	78.7
trnS-GCU-trnG-UCC_p4	CAATCCGACGCTTTAGTCCA	GGATCCATAGAACGAATCGCA	50.0	47.6	58.0	58.0	1180-1236 bp	1.0	77.5
trnS-GCU-trnG-UCC_p5	AATCCGACGCTTTAGTCCAC	GGATCCATAGAACGAATCGCA	50.0	47.6	58.0	58.0	1179-1235 bp	1.0	77.5