Hotspot

trnK-UUU-rps16

LSC · 4463-6155 · 1693 bp

Back to Pithecellobium

Pi 0.0948

MarkerSeek score 80.20

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 1693
variable_sites: 141
pi: 0.0948
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CCTTTCTGGATCAGTCGTGG	AAAAAGGCGCTCAACCTACA	55.0	45.0	58.0	58.0	1886-1928 bp	1.0	89.7
trnK-UUU-rps16_p2	CCTTTCTGGATCAGTCGTGG	CCCAATGAGCCGTTTATCGA	55.0	50.0	58.0	58.1	2024-2066 bp	1.0	89.1
trnK-UUU-rps16_p3	CCTTTCTGGATCAGTCGTGG	TCCCAATGAGCCGTTTATCG	55.0	50.0	58.0	58.1	2025-2067 bp	1.0	89.1
trnK-UUU-rps16_p4	CCTTTCTGGATCAGTCGTGG	TGAGGTTGCGAATACGAGTG	55.0	50.0	58.0	58.0	2235-2276 bp	1.0	89.0
trnK-UUU-rps16_p5	CCTTTCTGGATCAGTCGTGG	AAGCTAACGGATCGTGGAAC	55.0	50.0	58.0	58.0	2446-2486 bp	1.0	88.6