Hotspot

rrn16

IRb · 96679-98169 · 1491 bp

Back to Pitcairnia

Pi 0
MarkerSeek score 40.00
Primer available yes
Label rrn16

Feature Metadata

label_name
rrn16
feature_type
rRNA
region
IRb
length_bp
1491
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn16_p1 CCAGGCTAATATGAAGCGCA CAGACGTAGCTCGCTTCTTT 50.0 50.0 58.1 58.0 1688 bp 1.0 78.9
rrn16_p2 CCAGGCTAATATGAAGCGCA TCGCTTCTTTTTGGGTGTGA 50.0 45.0 58.1 58.0 1678 bp 1.0 78.7
rrn16_p3 TCCAGGCTAATATGAAGCGC CAGACGTAGCTCGCTTCTTT 50.0 50.0 57.8 58.0 1689 bp 1.0 78.5
rrn16_p4 TCCAGGCTAATATGAAGCGC TCGCTTCTTTTTGGGTGTGA 50.0 45.0 57.8 58.0 1679 bp 1.0 78.3
rrn16_p5 CCAGGCTAATATGAAGCGCA TGTGAAGCAGTGTCAAACCA 50.0 45.0 58.1 57.9 1663 bp 1.0 78.1