Hotspot

atpF

LSC · 3873-5334 · 1462 bp

Back to Pitcairnia

Pi 0.01

MarkerSeek score 61.70

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1462
variable_sites: 14
pi: 0.01
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACTAATTTCGTCGGCTCGAA	GGGCGAGCGAAGTGATATAA	45.0	50.0	57.4	57.6	1605-1636 bp	1.0	75.3
atpF_p2	GGCTCGAAGGGTTACCATTA	GGGCGAGCGAAGTGATATAA	50.0	50.0	57.0	57.6	1593-1624 bp	1.0	72.5
atpF_p3	TCTTTTTCGGAAGCAAAGGGA	GGGCGAGCGAAGTGATATAA	42.9	50.0	58.1	57.6	1561-1592 bp	1.0	72.0
atpF_p4	GGCTCGAAGGGTTACCATTAG	GGGCGAGCGAAGTGATATAA	52.4	50.0	58.2	57.6	1593-1624 bp	1.0	71.1
atpF_p5	ACTAATTTCGTCGGCTCGAA	GGGCGAGCGAAGTGATATAAA	45.0	47.6	57.4	58.2	1605-1636 bp	1.0	68.8