Hotspot

rrn16-trnI-GAU

IRb · 98170-98485 · 316 bp

Back to Pitcairnia

Pi 0.045
MarkerSeek score 80.20
Primer available yes
Label rrn16-trnI-GAU

Feature Metadata

label_name
rrn16-trnI-GAU
feature_type
igs
region
IRb
length_bp
316
variable_sites
14
pi
0.045
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn16-trnI-GAU_p1 CAAGGTAGCCGTACTGGAAG CTATCCATGTGGCTGAGAGC 55.0 55.0 57.7 57.8 437-442 bp 1.0 79.8
rrn16-trnI-GAU_p2 CAAGGTAGCCGTACTGGAAG GGCACAACGACGCAATTATC 55.0 50.0 57.7 58.2 405-410 bp 1.0 79.8
rrn16-trnI-GAU_p3 CAAGGTAGCCGTACTGGAAG CGCTGATGAGCACATTGAAC 55.0 50.0 57.7 57.8 456-461 bp 1.0 79.6
rrn16-trnI-GAU_p4 AGTGACTGGAGTGAAGTCGT CTATCCATGTGGCTGAGAGC 50.0 55.0 58.3 57.8 459-464 bp 1.0 79.5
rrn16-trnI-GAU_p5 AGTGACTGGAGTGAAGTCGT CGCTGATGAGCACATTGAAC 50.0 50.0 58.3 57.8 478-483 bp 1.0 79.4