Hotspot

psaJ-rpl33

LSC · 62496-63029 · 534 bp

Back to Pitcairnia

Pi 0.0131

MarkerSeek score 52.40

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 534
variable_sites: 7
pi: 0.0131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GCCCTGTCATTCCCCTTTTT	CCCGCGATTCCTTATTGACA	50.0	50.0	58.4	58.1	649-671 bp	1.0	77.6
psaJ-rpl33_p2	GCCCTGTCATTCCCCTTTTT	ATATCTAGAAATGCCCCGCG	50.0	50.0	58.4	57.6	663-685 bp	1.0	74.9
psaJ-rpl33_p3	GCCCTGTCATTCCCCTTTTT	GACATCTTTACCCTTGGCCA	50.0	50.0	58.4	57.5	585-607 bp	1.0	74.0
psaJ-rpl33_p4	GCCCTGTCATTCCCCTTTTT	TGACATCTTTACCCTTGGCC	50.0	50.0	58.4	57.5	586-608 bp	1.0	74.0
psaJ-rpl33_p5	GCCCTGTCATTCCCCTTTTT	TATCTAGAAATGCCCCGCGA	50.0	50.0	58.4	58.7	662-684 bp	1.0	72.7