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psbE-petL

LSC · 59646-61194 · 1549 bp

Back to Pitcairnia

Pi 0.00788

MarkerSeek score 60.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1549
variable_sites: 12
pi: 0.00788
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCAGCCAGTATAAAACCGA	50.0	45.0	57.9	56.9	1675-1695 bp	1.0	72.8
psbE-petL_p2	ACTGACGAATAACCAACCCG	AGCAGCCAGTATAAAACCGA	50.0	45.0	57.9	56.9	1688-1708 bp	1.0	72.5
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AGCAGCCAGTATAAAACCGA	45.0	45.0	58.2	56.9	1684-1704 bp	1.0	71.9
psbE-petL_p4	CGAATAACCAACCCGCAATG	AGCAGCCAGTATAAAACCGA	50.0	45.0	57.8	56.9	1683-1703 bp	1.0	71.7
psbE-petL_p5	ACCAACCCGCAATGAATAGG	AGCAGCCAGTATAAAACCGA	50.0	45.0	58.2	56.9	1677-1697 bp	1.0	71.3