Hotspot

petA-psbJ

LSC · 57755-58876 · 1122 bp

Back to Pitcairnia

Pi 0.00802
MarkerSeek score 62.90
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1122
variable_sites
9
pi
0.00802
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GGCCTTTTGTTCTTCTTCGC TCTGGGTTGGGTTCATCTCT 50.0 50.0 57.9 58.0 1248 bp 1.0 79.8
petA-psbJ_p2 GGCCTTTTGTTCTTCTTCGC TGGGTTGGGTTCATCTCTCT 50.0 50.0 57.9 58.0 1246 bp 1.0 79.8
petA-psbJ_p3 GGCCTTTTGTTCTTCTTCGC CTGGGTTGGGTTCATCTCTC 50.0 55.0 57.9 57.6 1247 bp 1.0 76.9
petA-psbJ_p4 CGTCCAAGGCCTTTTGTTCT TCTGGGTTGGGTTCATCTCT 50.0 50.0 58.7 58.0 1255 bp 1.0 74.8
petA-psbJ_p5 CGTCCAAGGCCTTTTGTTCT TGGGTTGGGTTCATCTCTCT 50.0 50.0 58.7 58.0 1253 bp 1.0 74.8