Hotspot

rbcL-accD

LSC · 50822-51582 · 761 bp

Back to Pitcairnia

Pi 0.00526

MarkerSeek score 61.80

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 761
variable_sites: 4
pi: 0.00526
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CTAGCAAATGGAGCCCTGAG	GGGTTCATCACTTCCACTGG	55.0	55.0	58.0	58.2	979-981 bp	1.0	78.7
rbcL-accD_p2	TGAAGCTAGCAAATGGAGCC	GGGTTCATCACTTCCACTGG	50.0	55.0	58.2	58.2	984-986 bp	1.0	77.0
rbcL-accD_p3	TCGACTTCGAACCGGTAGAT	GGGTTCATCACTTCCACTGG	50.0	55.0	58.3	58.2	922-924 bp	1.0	76.9
rbcL-accD_p4	CCGTGAAGCTAGCAAATGGA	GGGTTCATCACTTCCACTGG	50.0	55.0	58.3	58.2	987-989 bp	1.0	76.8
rbcL-accD_p5	TCCGTGAAGCTAGCAAATGG	GGGTTCATCACTTCCACTGG	50.0	55.0	58.3	58.2	988-990 bp	1.0	76.8