Hotspot

psaA-ycf3

LSC · 35672-36329 · 658 bp

Back to Pitcairnia

Pi 0.00308

MarkerSeek score 61.80

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 658
variable_sites: 2
pi: 0.00308
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	GTTGAAAATCACGAAGCGCT	50.0	45.0	57.9	57.7	757-765 bp	1.0	77.9
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	GCTATAGCGCTTACTCCAGG	50.0	55.0	57.9	57.6	801-809 bp	1.0	77.5
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	CCGATCAAGCTGCTGAGTAT	50.0	50.0	57.9	57.5	830-838 bp	1.0	76.1
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	TCCGATCAAGCTGCTGAGTA	50.0	50.0	57.9	58.5	831-839 bp	1.0	76.1
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	TGAAAATCACGAAGCGCTTC	50.0	45.0	57.9	57.4	755-763 bp	1.0	75.6