Hotspot

trnT-GGU-psbD

LSC · 25766-26783 · 1018 bp

Back to Pitcairnia

Pi 0.00787
MarkerSeek score 63.40
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1018
variable_sites
8
pi
0.00787
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GGACCAGCCTACAAAAACGA 55.0 50.0 58.1 58.1 1178-1182 bp 1.0 79.2
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GGACCAGCCTACAAAAACGA 50.0 50.0 58.1 58.1 1181-1185 bp 1.0 79.0
trnT-GGU-psbD_p3 ACGGCCCTTTTAACTCAGTG GGACCAGCCTACAAAAACGA 50.0 50.0 58.1 58.1 1195-1199 bp 1.0 78.8
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG ACAAAAACGAAACGGTCCCT 55.0 45.0 58.1 58.2 1168-1172 bp 1.0 78.1
trnT-GGU-psbD_p5 TCAGTGGTAGAGTAACGCCA ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1171-1175 bp 1.0 78.0