primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	atpF	1	ACTAATTTCGTCGGCTCGAA	GGGCGAGCGAAGTGATATAA	20	20	45.0	50.0	57.4	57.6	9.6	0.0	1.076007	3873	5334	1605-1636	1.000000	75.3
atpF_p2	atpF	2	GGCTCGAAGGGTTACCATTA	GGGCGAGCGAAGTGATATAA	20	20	50.0	50.0	57.0	57.6	0.0	0.0	1.428054	3873	5334	1593-1624	1.000000	72.5
atpF_p3	atpF	3	TCTTTTTCGGAAGCAAAGGGA	GGGCGAGCGAAGTGATATAA	21	20	42.9	50.0	58.1	57.6	12.6	0.0	1.495030	3873	5334	1561-1592	1.000000	72.0
atpF_p4	atpF	4	GGCTCGAAGGGTTACCATTAG	GGGCGAGCGAAGTGATATAA	21	20	52.4	50.0	58.2	57.6	0.0	0.0	1.598841	3873	5334	1593-1624	1.000000	71.1
atpF_p5	atpF	5	ACTAATTTCGTCGGCTCGAA	GGGCGAGCGAAGTGATATAAA	20	21	45.0	47.6	57.4	58.2	9.6	0.0	1.891191	3873	5334	1605-1636	1.000000	68.8
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	20	20	45.0	55.0	58.0	57.8	1.4	21.3	0.214265	6026	6987	1019-1075	1.000000	79.7
atpH-atpI_p2	atpH-atpI	2	TACCTTGACCAACTCCAGGT	CGAATCCATGGAGGGTCATC	20	20	50.0	55.0	57.9	57.8	0.0	21.3	0.288157	6026	6987	1075-1131	1.000000	79.0
atpH-atpI_p3	atpH-atpI	3	AGCCAATCCAGCAGCAATAA	CGAATCCATGGAGGGTCATC	20	20	45.0	55.0	57.9	57.8	0.0	21.3	0.324323	6026	6987	1035-1091	1.000000	78.8
atpH-atpI_p4	atpH-atpI	4	AATAACGGAAGCGGCAGAAA	CGAATCCATGGAGGGTCATC	20	20	45.0	55.0	58.2	57.8	1.4	21.3	0.359803	6026	6987	1020-1076	1.000000	78.5
atpH-atpI_p5	atpH-atpI	5	GAAGCAAGTCCTACAGCCAA	CGAATCCATGGAGGGTCATC	20	20	50.0	55.0	57.8	57.8	0.0	21.3	0.360882	6026	6987	1049-1105	1.000000	78.4
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	TCCCTCATTTCCATTCCGGA	GCGACACCCAGATTTGAACT	20	20	50.0	50.0	58.4	58.5	0.0	0.0	0.885652	19317	20632	1466-1466	1.000000	73.4
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	TTCCCTCATTTCCATTCCGG	GCGACACCCAGATTTGAACT	20	20	50.0	50.0	57.6	58.5	0.0	0.0	0.911615	19317	20632	1467-1467	1.000000	73.2
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TCCCTCATTTCCATTCCGGA	TTTGTTGATCAGGCGACACC	20	20	50.0	50.0	58.4	58.8	0.0	0.0	1.174588	19317	20632	1478-1478	1.000000	71.1
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	TTCCCTCATTTCCATTCCGG	TTTGTTGATCAGGCGACACC	20	20	50.0	50.0	57.6	58.8	0.0	0.0	1.200551	19317	20632	1479-1479	1.000000	70.9
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	TCCCTCATTTCCATTCCGGA	TTTTGTTGATCAGGCGACAC	20	20	50.0	45.0	58.4	56.9	0.0	0.0	1.466506	19317	20632	1479-1479	1.000000	68.8
trnC-GCA-petN_p1	trnC-GCA-petN	1	AGTTCAAATCTGGGTGTCGC	AGTCCACTCCTTCCCCATAC	20	20	50.0	55.0	58.5	58.1	0.0	0.0	0.595302	20704	21755	1140-1160	1.000000	77.2
trnC-GCA-petN_p2	trnC-GCA-petN	2	AGTTCAAATCTGGGTGTCGC	GTCCACTCCTTCCCCATACT	20	20	50.0	55.0	58.5	58.1	0.0	0.0	0.595302	20704	21755	1139-1159	1.000000	77.2
trnC-GCA-petN_p3	trnC-GCA-petN	3	AGTTCAAATCTGGGTGTCGC	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.5	58.2	0.0	0.0	0.627590	20704	21755	1081-1101	1.000000	77.0
trnC-GCA-petN_p4	trnC-GCA-petN	4	AGTTCAAATCTGGGTGTCGC	GAGTCCACTCCTTCCCCATA	20	20	50.0	55.0	58.5	57.8	0.0	0.0	0.652490	20704	21755	1141-1161	1.000000	76.7
trnC-GCA-petN_p5	trnC-GCA-petN	5	AGTTCAAATCTGGGTGTCGC	CTAGAGTCCACTCCTTCCCC	20	20	50.0	60.0	58.5	58.2	0.0	3.0	0.693171	20704	21755	1144-1164	1.000000	76.4
trnY-GUA-trnE-UUC_p1	trnY-GUA-trnE-UUC	1	TGGAGCGGTGAATTATTGGG	ATGACGGTTGGGCGTATATG	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.215152	24805	24863	251-251	1.000000	78.9
trnY-GUA-trnE-UUC_p2	trnY-GUA-trnE-UUC	2	TGGAGCGGTGAATTATTGGG	GCGTATATGCCCCTATCGTC	20	20	50.0	55.0	57.9	57.8	0.0	5.6	0.256641	24805	24863	240-240	1.000000	78.6
trnY-GUA-trnE-UUC_p3	trnY-GUA-trnE-UUC	3	TGGAGCGGTGAATTATTGGG	CTCTCTTTCAAGGAGGCAGC	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.314723	24805	24863	204-204	1.000000	78.3
trnY-GUA-trnE-UUC_p4	trnY-GUA-trnE-UUC	4	TGGAGCGGTGAATTATTGGG	TCGTCTAGTGGTTCAGGACA	20	20	50.0	50.0	57.9	57.7	0.0	0.0	0.332873	24805	24863	225-225	1.000000	78.0
trnY-GUA-trnE-UUC_p5	trnY-GUA-trnE-UUC	5	CCAGCGTAGACATATTGCCA	ATGACGGTTGGGCGTATATG	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.402760	24805	24863	215-215	1.000000	77.5
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	20	20	55.0	50.0	58.1	58.1	7.6	0.0	0.177181	25766	26783	1178-1182	1.000000	79.2
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.199336	25766	26783	1181-1185	1.000000	79.0
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	ACGGCCCTTTTAACTCAGTG	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.220904	25766	26783	1195-1199	1.000000	78.8
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GTGGTAGAGTAACGCCATGG	ACAAAAACGAAACGGTCCCT	20	20	55.0	45.0	58.1	58.2	7.6	0.0	0.310137	25766	26783	1168-1172	1.000000	78.1
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	TCAGTGGTAGAGTAACGCCA	ACAAAAACGAAACGGTCCCT	20	20	50.0	45.0	58.1	58.2	0.0	0.0	0.332292	25766	26783	1171-1175	1.000000	78.0
psaA-ycf3_p1	psaA-ycf3	1	CCCATTCCTCGAAAGACGTT	GTTGAAAATCACGAAGCGCT	20	20	50.0	45.0	57.9	57.7	0.0	2.6	0.410881	35672	36329	757-765	1.000000	77.9
psaA-ycf3_p2	psaA-ycf3	2	CCCATTCCTCGAAAGACGTT	GCTATAGCGCTTACTCCAGG	20	20	50.0	55.0	57.9	57.6	0.0	18.7	0.450479	35672	36329	801-809	1.000000	77.5
psaA-ycf3_p3	psaA-ycf3	3	CCCATTCCTCGAAAGACGTT	CCGATCAAGCTGCTGAGTAT	20	20	50.0	50.0	57.9	57.5	0.0	1.4	0.617598	35672	36329	830-838	1.000000	76.1
psaA-ycf3_p4	psaA-ycf3	4	CCCATTCCTCGAAAGACGTT	TCCGATCAAGCTGCTGAGTA	20	20	50.0	50.0	57.9	58.5	0.0	1.4	0.620433	35672	36329	831-839	1.000000	76.1
psaA-ycf3_p5	psaA-ycf3	5	CCCATTCCTCGAAAGACGTT	TGAAAATCACGAAGCGCTTC	20	20	50.0	45.0	57.9	57.4	0.0	35.0	0.689291	35672	36329	755-763	1.000000	75.6
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	AGAGAAAAAGCCGGCTATCG	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	58.1	58.0	33.1	0.0	0.063299	40307	41119	914-928	1.000000	81.0
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	40307	41119	863-877	1.000000	80.5
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	AGAGAAAAAGCCGGCTATCG	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	58.1	58.1	33.1	0.0	0.128108	40307	41119	922-936	1.000000	80.4
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GAGTCGAACCGATGACCATC	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	58.2	58.0	0.0	0.0	0.163174	40307	41119	894-908	1.000000	80.2
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	40307	41119	871-885	1.000000	79.9
rbcL-accD_p1	rbcL-accD	1	CTAGCAAATGGAGCCCTGAG	GGGTTCATCACTTCCACTGG	20	20	55.0	55.0	58.0	58.2	0.0	0.0	0.209063	50822	51582	979-981	1.000000	78.7
rbcL-accD_p2	rbcL-accD	2	TGAAGCTAGCAAATGGAGCC	GGGTTCATCACTTCCACTGG	20	20	50.0	55.0	58.2	58.2	18.8	0.0	0.418436	50822	51582	984-986	1.000000	77.0
rbcL-accD_p3	rbcL-accD	3	TCGACTTCGAACCGGTAGAT	GGGTTCATCACTTCCACTGG	20	20	50.0	55.0	58.3	58.2	8.0	0.0	0.430854	50822	51582	922-924	1.000000	76.9
rbcL-accD_p4	rbcL-accD	4	CCGTGAAGCTAGCAAATGGA	GGGTTCATCACTTCCACTGG	20	20	50.0	55.0	58.3	58.2	18.8	0.0	0.435777	50822	51582	987-989	1.000000	76.8
rbcL-accD_p5	rbcL-accD	5	TCCGTGAAGCTAGCAAATGG	GGGTTCATCACTTCCACTGG	20	20	50.0	55.0	58.3	58.2	18.8	0.0	0.435777	50822	51582	988-990	1.000000	76.8
accD-psaI_p1	accD-psaI	1	AGCTCCATGGTTTCTTTCCC	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.8	58.0	12.6	26.9	0.249230	53050	54034	1099-1149	1.000000	79.2
accD-psaI_p2	accD-psaI	2	GCTCCATGGTTTCTTTCCCT	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.8	58.0	12.6	26.9	0.249230	53050	54034	1098-1148	1.000000	79.2
accD-psaI_p3	accD-psaI	3	AGCTCCATGGTTTCTTTCCC	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.8	57.9	12.6	26.8	0.325764	53050	54034	1105-1155	1.000000	78.6
accD-psaI_p4	accD-psaI	4	GCTCCATGGTTTCTTTCCCT	AAGAAGCCATTGCAATTGCC	20	20	50.0	45.0	57.8	57.9	12.6	26.8	0.325764	53050	54034	1104-1154	1.000000	78.6
accD-psaI_p5	accD-psaI	5	GACCCAATCGTACCACGTAA	CCATTGCAATTGCCGGAAAT	20	20	50.0	45.0	57.3	58.0	0.0	26.9	0.679944	53050	54034	1151-1201	1.000000	75.7
petA-psbJ_p1	petA-psbJ	1	GGCCTTTTGTTCTTCTTCGC	TCTGGGTTGGGTTCATCTCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.096671	57755	58876	1248-1248	1.000000	79.8
petA-psbJ_p2	petA-psbJ	2	GGCCTTTTGTTCTTCTTCGC	TGGGTTGGGTTCATCTCTCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.096671	57755	58876	1246-1246	1.000000	79.8
petA-psbJ_p3	petA-psbJ	3	GGCCTTTTGTTCTTCTTCGC	CTGGGTTGGGTTCATCTCTC	20	20	50.0	55.0	57.9	57.6	0.0	0.0	0.461827	57755	58876	1247-1247	1.000000	76.9
petA-psbJ_p4	petA-psbJ	4	CGTCCAAGGCCTTTTGTTCT	TCTGGGTTGGGTTCATCTCT	20	20	50.0	50.0	58.7	58.0	17.6	0.0	0.726216	57755	58876	1255-1255	1.000000	74.8
petA-psbJ_p5	petA-psbJ	5	CGTCCAAGGCCTTTTGTTCT	TGGGTTGGGTTCATCTCTCT	20	20	50.0	50.0	58.7	58.0	17.6	0.0	0.726216	57755	58876	1253-1253	1.000000	74.8
psbE-petL_p1	psbE-petL	1	CAACCCGCAATGAATAGGGA	AGCAGCCAGTATAAAACCGA	20	20	50.0	45.0	57.9	56.9	6.4	0.0	1.123272	59646	61194	1675-1695	1.000000	72.8
psbE-petL_p2	psbE-petL	2	ACTGACGAATAACCAACCCG	AGCAGCCAGTATAAAACCGA	20	20	50.0	45.0	57.9	56.9	0.0	0.0	1.160887	59646	61194	1688-1708	1.000000	72.5
psbE-petL_p3	psbE-petL	3	ACGAATAACCAACCCGCAAT	AGCAGCCAGTATAAAACCGA	20	20	45.0	45.0	58.2	56.9	0.0	0.0	1.242959	59646	61194	1684-1704	1.000000	71.9
psbE-petL_p4	psbE-petL	4	CGAATAACCAACCCGCAATG	AGCAGCCAGTATAAAACCGA	20	20	50.0	45.0	57.8	56.9	0.0	0.0	1.259289	59646	61194	1683-1703	1.000000	71.7
psbE-petL_p5	psbE-petL	5	ACCAACCCGCAATGAATAGG	AGCAGCCAGTATAAAACCGA	20	20	50.0	45.0	58.2	56.9	0.0	0.0	1.309230	59646	61194	1677-1697	1.000000	71.3
psaJ-rpl33_p1	psaJ-rpl33	1	GCCCTGTCATTCCCCTTTTT	CCCGCGATTCCTTATTGACA	20	20	50.0	50.0	58.4	58.1	0.0	8.2	0.407387	62496	63029	649-671	1.000000	77.6
psaJ-rpl33_p2	psaJ-rpl33	2	GCCCTGTCATTCCCCTTTTT	ATATCTAGAAATGCCCCGCG	20	20	50.0	50.0	58.4	57.6	0.0	0.0	0.742152	62496	63029	663-685	1.000000	74.9
psaJ-rpl33_p3	psaJ-rpl33	3	GCCCTGTCATTCCCCTTTTT	GACATCTTTACCCTTGGCCA	20	20	50.0	50.0	58.4	57.5	0.0	0.0	0.862898	62496	63029	585-607	1.000000	74.0
psaJ-rpl33_p4	psaJ-rpl33	4	GCCCTGTCATTCCCCTTTTT	TGACATCTTTACCCTTGGCC	20	20	50.0	50.0	58.4	57.5	0.0	0.0	0.862898	62496	63029	586-608	1.000000	74.0
psaJ-rpl33_p5	psaJ-rpl33	5	GCCCTGTCATTCCCCTTTTT	TATCTAGAAATGCCCCGCGA	20	20	50.0	50.0	58.4	58.7	0.0	8.2	1.021331	62496	63029	662-684	1.000000	72.7
rps11_p1	rps11	1	GGGTTCCATACGATATCCGC	GGATCCGGCGCAGTTTTATA	20	20	55.0	50.0	58.0	58.1	0.0	10.8	0.132273	74590	75006	1459-1460	1.000000	79.7
rps11_p2	rps11	2	GGGTTCCATACGATATCCGC	GCGTGGGCGAATTATAGTGA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.145295	74590	75006	1177-1178	1.000000	79.7
rps11_p3	rps11	3	TCTAGGACCCCTTACGCAAA	GGATCCGGCGCAGTTTTATA	20	20	50.0	50.0	58.1	58.1	0.0	10.8	0.184925	74590	75006	1318-1319	1.000000	79.3
rps11_p4	rps11	4	GGGTTCCATACGATATCCGC	ACTGAATCACTTCCCAACGG	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.190975	74590	75006	1535-1536	1.000000	79.1
rps11_p5	rps11	5	GGGTTCCATACGATATCCGC	CTGAATCACTTCCCAACGGT	20	20	55.0	50.0	58.0	57.8	0.0	0.0	0.190975	74590	75006	1534-1535	1.000000	79.1
rps11-rpl36_p1	rps11-rpl36	1	ACGCCCATTCCTATGTGAAC	GCGTGGGCGAATTATAGTGA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.177711	75007	75158	254-255	1.000000	82.0
rps11-rpl36_p2	rps11-rpl36	2	GAACCAATACGCCCATTCCT	GCGTGGGCGAATTATAGTGA	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.192106	75007	75158	262-263	1.000000	81.8
rps11-rpl36_p3	rps11-rpl36	3	ACGCCCATTCCTATGTGAAC	AAGGGCTTCCGTTCGTAAAA	20	20	50.0	45.0	58.0	57.7	0.0	0.0	0.297653	75007	75158	302-303	1.000000	80.5
rps11-rpl36_p4	rps11-rpl36	4	GAACCAATACGCCCATTCCT	AAGGGCTTCCGTTCGTAAAA	20	20	50.0	45.0	57.9	57.7	0.0	0.0	0.312049	75007	75158	310-311	1.000000	80.3
rps11-rpl36_p5	rps11-rpl36	5	TGAACCAATACGCCCATTCC	GCGTGGGCGAATTATAGTGA	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.381642	75007	75158	263-264	1.000000	80.3
rrn16_p1	rrn16	1	CCAGGCTAATATGAAGCGCA	CAGACGTAGCTCGCTTCTTT	20	20	50.0	50.0	58.1	58.0	11.5	0.0	0.133115	96679	98169	1688-1688	1.000000	78.9
rrn16_p2	rrn16	2	CCAGGCTAATATGAAGCGCA	TCGCTTCTTTTTGGGTGTGA	20	20	50.0	45.0	58.1	58.0	11.5	0.0	0.162649	96679	98169	1678-1678	1.000000	78.7
rrn16_p3	rrn16	3	TCCAGGCTAATATGAAGCGC	CAGACGTAGCTCGCTTCTTT	20	20	50.0	50.0	57.8	58.0	0.0	0.0	0.185860	96679	98169	1689-1689	1.000000	78.5
rrn16_p4	rrn16	4	TCCAGGCTAATATGAAGCGC	TCGCTTCTTTTTGGGTGTGA	20	20	50.0	45.0	57.8	58.0	0.0	0.0	0.215394	96679	98169	1679-1679	1.000000	78.3
rrn16_p5	rrn16	5	CCAGGCTAATATGAAGCGCA	TGTGAAGCAGTGTCAAACCA	20	20	50.0	45.0	58.1	57.9	11.5	0.0	0.238379	96679	98169	1663-1663	1.000000	78.1
rrn16-trnI-GAU_p1	rrn16-trnI-GAU	1	CAAGGTAGCCGTACTGGAAG	CTATCCATGTGGCTGAGAGC	20	20	55.0	55.0	57.7	57.8	7.8	0.0	0.462074	98170	98485	437-442	1.000000	79.8
rrn16-trnI-GAU_p2	rrn16-trnI-GAU	2	CAAGGTAGCCGTACTGGAAG	GGCACAACGACGCAATTATC	20	20	55.0	50.0	57.7	58.2	7.8	0.0	0.485056	98170	98485	405-410	1.000000	79.8
rrn16-trnI-GAU_p3	rrn16-trnI-GAU	3	CAAGGTAGCCGTACTGGAAG	CGCTGATGAGCACATTGAAC	20	20	55.0	50.0	57.7	57.8	7.8	4.7	0.464916	98170	98485	456-461	1.000000	79.6
rrn16-trnI-GAU_p4	rrn16-trnI-GAU	4	AGTGACTGGAGTGAAGTCGT	CTATCCATGTGGCTGAGAGC	20	20	50.0	55.0	58.3	57.8	0.0	0.0	0.473195	98170	98485	459-464	1.000000	79.5
rrn16-trnI-GAU_p5	rrn16-trnI-GAU	5	AGTGACTGGAGTGAAGTCGT	CGCTGATGAGCACATTGAAC	20	20	50.0	50.0	58.3	57.8	0.0	4.7	0.476037	98170	98485	478-483	1.000000	79.4