Hotspot

ccsA-ndhD

SSC · 120864-121239 · 376 bp

Back to Pistacia

Pi 0.0123

MarkerSeek score 64.70

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 376
variable_sites: 14
pi: 0.0123
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGGATATGCTACTTCGGGGT	AGTGGACAAGGTCGAAGCTA	50.0	50.0	57.9	58.4	466-501 bp	1.0	82.2
ccsA-ndhD_p2	TGTGGCTTCTATGGGCTTTC	AGTGGACAAGGTCGAAGCTA	50.0	50.0	57.9	58.4	494-529 bp	1.0	81.6
ccsA-ndhD_p3	TGGATATGCTACTTCGGGGT	CCTATCAGTGGACAAGGTCG	50.0	55.0	57.9	57.4	472-507 bp	1.0	80.4
ccsA-ndhD_p4	TGGATATGCTACTTCGGGGT	TCCCTATCAGTGGACAAGGT	50.0	50.0	57.9	57.4	474-509 bp	1.0	79.9
ccsA-ndhD_p5	TGTGGCTTCTATGGGCTTTC	CCTATCAGTGGACAAGGTCG	50.0	55.0	57.9	57.4	500-535 bp	1.0	79.8