Hotspot

rpl32-trnL-UAG

SSC · 118478-119702 · 1225 bp

Back to Pistacia

Pi 0.00428

MarkerSeek score 56.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1225
variable_sites: 16
pi: 0.00428
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TAGCGAAATCCCTTTCCACG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1315-1361 bp	1.0	81.7
rpl32-trnL-UAG_p2	TAGCGAAATCCCTTTCCACG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1316-1362 bp	1.0	81.6
rpl32-trnL-UAG_p3	TCCACGGGGAATTCACAAAG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	1301-1347 bp	1.0	81.1
rpl32-trnL-UAG_p4	TAGCGAAATCCCTTTCCACG	TCGAACCGAGATGCTTTAGC	50.0	50.0	58.0	58.1	1351-1397 bp	1.0	81.0
rpl32-trnL-UAG_p5	TCCACGGGGAATTCACAAAG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.1	58.0	1302-1348 bp	1.0	81.0