Hotspot

rps19-rpl2

LSC · 88381-88613 · 233 bp

Back to Pistacia

Pi 0.0108

MarkerSeek score 36.70

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: LSC
length_bp: 233
variable_sites: 8
pi: 0.0108
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TAGCAATAGTATGGCCGACC	CTTCGCCGCCGTAGTAAATA	50.0	50.0	56.9	57.9	276-289 bp	1.0	72.3
rps19-rpl2_p2	TAGCAATAGTATGGCCGACC	TTCGCCGCCGTAGTAAATAG	50.0	50.0	56.9	57.9	275-288 bp	1.0	72.3
rps19-rpl2_p3	TAGCAATAGTATGGCCGACC	TAGAAAAAGACCCGCAACCC	50.0	50.0	56.9	58.1	361-374 bp	1.0	72.0
rps19-rpl2_p4	TAGCAATAGTATGGCCGACC	TTGGGGTTATCCTGCACTTG	50.0	50.0	56.9	57.8	340-353 bp	1.0	71.2
rps19-rpl2_p5	TAGCAATAGTATGGCCGACC	GGGTTATCCTGCACTTGGAA	50.0	50.0	56.9	57.5	337-350 bp	1.0	68.8