Hotspot

petD-rpoA

LSC · 81864-82144 · 281 bp

Back to Pistacia

Pi 0.0269

MarkerSeek score 62.20

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 281
variable_sites: 21
pi: 0.0269
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GCTTGGGTATTGGAGCAACA	TCGCATAGAAGATGTAAAACAGA	50.0	34.8	58.5	55.8	385-390 bp	1.0	45.6
petD-rpoA_p2	TGCTTGGGTATTGGAGCAAC	TCGCATAGAAGATGTAAAACAGA	50.0	34.8	58.5	55.8	386-391 bp	1.0	45.6
petD-rpoA_p3	CCTGTGCTTGGGTATTGGAG	TCGCATAGAAGATGTAAAACAGA	55.0	34.8	58.2	55.8	390-395 bp	1.0	45.5
petD-rpoA_p4	CCAGTAGCAACAACCGTCTT	TCGCATAGAAGATGTAAAACAGA	50.0	34.8	58.1	55.8	431-436 bp	1.0	45.0
petD-rpoA_p5	CCAGTAGCAACAACCGTCTT	TTCGCATAGAAGATGTAAAACAGA	50.0	33.3	58.1	56.5	432-437 bp	1.0	45.0