Hotspot

psaI-pafII

LSC · 62853-63309 · 457 bp

Back to Pistacia

Pi 0.00417

MarkerSeek score 54.70

Primer available yes

Label psaI-pafII

Feature Metadata

label_name: psaI-pafII
feature_type: igs
region: LSC
length_bp: 457
variable_sites: 6
pi: 0.00417
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-pafII_p1	ATTTCCGGCAATTGCAATGG	TTGTTTTGCGAGATCCTGCT	45.0	45.0	58.0	58.1	573-582 bp	1.0	80.2
psaI-pafII_p2	ATTTCCGGCAATTGCAATGG	GGATAAAGGCCCAGCAGAAA	45.0	50.0	58.0	57.9	597-606 bp	1.0	79.8
psaI-pafII_p3	ATTTCCGGCAATTGCAATGG	AAAGGATAAAGGCCCAGCAG	45.0	50.0	58.0	57.9	600-609 bp	1.0	79.8
psaI-pafII_p4	GGCAATTGCAATGGCTTCTT	TTGTTTTGCGAGATCCTGCT	45.0	45.0	57.9	58.1	567-576 bp	1.0	79.6
psaI-pafII_p5	GGCAATTGCAATGGCTTCTT	GGATAAAGGCCCAGCAGAAA	45.0	50.0	57.9	57.9	591-600 bp	1.0	79.2