Hotspot

trnV-UAC-trnM-CAU

LSC · 55266-55463 · 198 bp

Back to Pistacia

Pi 0.0111
MarkerSeek score 52.70
Primer available yes
Label trnV-UAC-trnM-CAU

Feature Metadata

label_name
trnV-UAC-trnM-CAU
feature_type
igs
region
LSC
length_bp
198
variable_sites
6
pi
0.0111
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-UAC-trnM-CAU_p1 TCTGAAAAACATTGGCGCAC CCCGCCGTATGAAAGCAATA 45.0 50.0 57.9 58.4 301 bp 1.0 77.2
trnV-UAC-trnM-CAU_p2 TCTGAAAAACATTGGCGCAC ATACCATTGACCGCGGTATC 45.0 50.0 57.9 57.5 360 bp 1.0 76.7
trnV-UAC-trnM-CAU_p3 TCTGAAAAACATTGGCGCAC GATACCATTGACCGCGGTAT 45.0 50.0 57.9 57.5 361 bp 1.0 76.7
trnV-UAC-trnM-CAU_p4 TCTGAAAAACATTGGCGCAC CCATTGACCGCGGTATCTAA 45.0 50.0 57.9 57.5 357 bp 1.0 76.1
trnV-UAC-trnM-CAU_p5 TCTGAAAAACATTGGCGCAC TTAGATACCATTGACCGCGG 45.0 50.0 57.9 57.5 364 bp 1.0 76.1