Pi
0.00601
MarkerSeek score
58.30
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 1049-1074 bp | 1.0 | 80.3 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 1057-1082 bp | 1.0 | 79.7 |
| trnT-UGU-trnL-UAA_p3 | GGAATCGAACCGATGACCAT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.8 | 58.0 | 1081-1106 bp | 1.0 | 79.4 |
| trnT-UGU-trnL-UAA_p4 | GGAATCGAACCGATGACCAT | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 57.8 | 58.1 | 1089-1114 bp | 1.0 | 78.8 |
| trnT-UGU-trnL-UAA_p5 | ACAGATAGAGAAAAGCCGGC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.7 | 58.0 | 1105-1109 bp | 0.857143 | 72.7 |