Hotspot

psaA-pafI

LSC · 45099-45796 · 698 bp

Back to Pistacia

Pi 0.00232

MarkerSeek score 52.50

Primer available yes

Label psaA-pafI

Feature Metadata

label_name: psaA-pafI
feature_type: igs
region: LSC
length_bp: 698
variable_sites: 4
pi: 0.00232
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-pafI_p1	GCCCATTCCTCGAAAGAAGT	TATCGCGCTTACTCCTGGTA	50.0	50.0	57.9	58.0	848-860 bp	1.0	79.2
psaA-pafI_p2	GCCCATTCCTCGAAAGAAGT	CGCGCTTACTCCTGGTAATT	50.0	50.0	57.9	58.1	845-857 bp	1.0	78.9
psaA-pafI_p3	GCCCATTCCTCGAAAGAAGT	AGCGCAGAATTGGTTGAAGA	50.0	45.0	57.9	58.1	818-830 bp	1.0	78.6
psaA-pafI_p4	GCCCATTCCTCGAAAGAAGT	ATTGGTTGAAGATCACGGGG	50.0	50.0	57.9	57.9	810-822 bp	1.0	78.4
psaA-pafI_p5	TTGAGAAATGACCCGGTCTG	TATCGCGCTTACTCCTGGTA	50.0	50.0	57.5	58.0	868-880 bp	1.0	76.4