Hotspot

psbM-trnD-GUC

LSC · 31429-32703 · 1275 bp

Back to Pistacia

Pi 0.00486

MarkerSeek score 57.50

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1275
variable_sites: 20
pi: 0.00486
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	TCCCCGGGATTGTAGTTCAA	45.0	50.0	55.8	58.3	1337-1401 bp	1.0	64.5
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	TTCCCCGGGATTGTAGTTCA	45.0	50.0	55.8	58.3	1338-1402 bp	1.0	64.5
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	1322-1386 bp	1.0	63.5
psbM-trnD-GUC_p4	AGGAACTAGAATGAACAGTGCA	TCCCCGGGATTGTAGTTCAA	40.9	50.0	57.4	58.3	1344-1408 bp	1.0	61.3
psbM-trnD-GUC_p5	AGGAACTAGAATGAACAGTGCA	TTCCCCGGGATTGTAGTTCA	40.9	50.0	57.4	58.3	1345-1409 bp	1.0	61.3