Hotspot

petN-psbM

LSC · 30619-31323 · 705 bp

Back to Pistacia

Pi 0.00567

MarkerSeek score 52.10

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 705
variable_sites: 12
pi: 0.00567
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	ACGTAAAAACTGTCAGTCAAGG	50.0	40.9	58.2	56.9	815-817 bp	1.0	55.1
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	CGTAAAAACTGTCAGTCAAGGT	50.0	40.9	58.2	56.9	814-816 bp	1.0	55.1
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	CGTAAAAACTGTCAGTCAAGGTG	50.0	43.5	58.2	58.2	814-816 bp	1.0	54.8
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	AAAACTGTCAGTCAAGGTGA	50.0	40.0	58.2	54.7	810-812 bp	1.0	54.1
petN-psbM_p5	ATGGGGAAGAAGTGGACTCT	CGTAAAAACTGTCAGTCAAGGT	50.0	40.9	57.6	56.9	753-755 bp	1.0	53.6