Hotspot

trnR-UCU-atpA

LSC · 11611-11814 · 204 bp

Back to Pistacia

Pi 0.0107

MarkerSeek score 43.40

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 204
variable_sites: 6
pi: 0.0107
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	GAGGAAGCAGAAGCCCTTTT	47.6	50.0	57.3	58.1	301 bp	1.0	67.4
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	CATTCACCGAGGAAGCAGAA	47.6	50.0	57.3	57.9	309 bp	1.0	67.2
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	AAGACATTCACCGAGGAAGC	47.6	50.0	57.3	57.9	313 bp	1.0	67.2
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	AGGAAGCAGAAGCCCTTTTG	47.6	50.0	57.3	58.4	300 bp	1.0	65.1
trnR-UCU-atpA_p5	GGTTCAAATCCTATTGGACGC	GGAAGCAGAAGCCCTTTTGA	47.6	50.0	57.3	58.4	299 bp	1.0	65.0