Hotspot

ccsA-ndhD

SSC · 112692-113025 · 334 bp

Back to Piptanthus

Pi 0.0223

MarkerSeek score 70.40

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 334
variable_sites: 7
pi: 0.0223
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TCAATAGTGGCCTCTCTAGG	CGGTTGACAAGGTTGAAGCT	50.0	50.0	55.4	58.7	476-480 bp	1.0	58.4
ccsA-ndhD_p2	TCAATAGTGGCCTCTCTAGG	TCGGTTGACAAGGTTGAAGC	50.0	50.0	55.4	58.7	477-481 bp	1.0	58.4
ccsA-ndhD_p3	CTTCAATAGTGGCCTCTCTAGG	CGGTTGACAAGGTTGAAGCT	50.0	50.0	57.4	58.7	478-482 bp	1.0	58.2
ccsA-ndhD_p4	CTTCAATAGTGGCCTCTCTAGG	TCGGTTGACAAGGTTGAAGC	50.0	50.0	57.4	58.7	479-483 bp	1.0	58.1
ccsA-ndhD_p5	TCTTCAATAGTGGCCTCTCT	CGGTTGACAAGGTTGAAGCT	45.0	50.0	55.2	58.7	479-483 bp	1.0	56.9