Hotspot

rps11-rpl36

LSC · 77028-77356 · 329 bp

Back to Piptanthus

Pi 0.00965

MarkerSeek score 63.30

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 329
variable_sites: 3
pi: 0.00965
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	CGTCCATTTTTACGCGAACC	GCGGGGGCGAATTATAGTAA	50.0	50.0	58.1	57.5	415-431 bp	1.0	79.3
rps11-rpl36_p2	TTACGCGAACCAATACGTCC	GCGGGGGCGAATTATAGTAA	50.0	50.0	58.4	57.5	430-446 bp	1.0	77.2
rps11-rpl36_p3	ACGTCCATTTTTACGCGAAC	GCGGGGGCGAATTATAGTAA	45.0	50.0	57.4	57.5	416-432 bp	1.0	75.6
rps11-rpl36_p4	CGTCCATTTTTACGCGAACC	AAGGGCCTCTGTTCGTAAAA	50.0	45.0	58.1	57.1	463-479 bp	1.0	75.0
rps11-rpl36_p5	CGTCCATTTTTACGCGAACC	GCGGGGGCGAATTATAGTAAT	50.0	47.6	58.1	58.0	415-431 bp	1.0	74.8