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petA-psbJ

LSC · 60356-61447 · 1092 bp

Back to Piptanthus

Pi 0.00641

MarkerSeek score 62.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1092
variable_sites: 7
pi: 0.00641
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CGTGTCCAAGGTCTCTTGTT	CATATTCCGGATTGGGCTCA	50.0	50.0	57.8	57.4	1232-1237 bp	1.0	74.0
petA-psbJ_p2	CGTTACGTGTCCAAGGTCTC	CATATTCCGGATTGGGCTCA	55.0	50.0	58.3	57.4	1237-1242 bp	1.0	73.5
petA-psbJ_p3	GATCCGTTACGTGTCCAAGG	CATATTCCGGATTGGGCTCA	55.0	50.0	58.4	57.4	1241-1246 bp	1.0	73.0
petA-psbJ_p4	CGTGTCCAAGGTCTCTTGTT	TATTCCGGATTGGGCTCATC	50.0	50.0	57.8	57.1	1230-1235 bp	1.0	71.7
petA-psbJ_p5	CGTGTCCAAGGTCTCTTGTT	TCATATTCCGGATTGGGCTC	50.0	50.0	57.8	57.1	1233-1238 bp	1.0	71.7