Hotspot

trnH-GUG-psbA

LSC · 77-267 · 191 bp

Back to Piptanthus

Pi 0.0419

MarkerSeek score 68.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 191
variable_sites: 8
pi: 0.0419
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	TAACCGGGCTAACCTTGGTA	55.0	50.0	58.1	58.1	313-374 bp	1.0	80.4
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	TAACCGGGCTAACCTTGGTA	50.0	50.0	57.9	58.1	327-388 bp	1.0	80.3
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	TAACCGGGCTAACCTTGGTA	50.0	50.0	57.9	58.1	326-387 bp	1.0	80.3
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	TAACCGGGCTAACCTTGGTA	50.0	50.0	57.9	58.1	328-389 bp	1.0	80.3
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	GGTCGAGGCTCCATCTCTAA	55.0	55.0	58.1	58.3	241-302 bp	1.0	78.9