Hotspot

ycf1

SSC · 131736-137198 · 5463 bp

Back to Piptadenia

Pi 0.384

MarkerSeek score 76.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5463
variable_sites: 1910
pi: 0.384
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTACAGAATTGGGAGCGCTC	GTCTGAAACCTTGGCACAGA	50.0	50.0	58.0	58.0	1158 bp	0.5	59.5
ycf1_p2	TTACAGAATTGGGAGCGCTC	TGCACCTATAATGGCGTTCC	50.0	50.0	58.0	58.0	1249 bp	0.5	59.5
ycf1_p3	TTACAGAATTGGGAGCGCTC	GCTACGATCCCCTCAAAAGG	50.0	55.0	58.0	58.0	1127 bp	0.5	59.4
ycf1_p4	TTACAGAATTGGGAGCGCTC	TTTCGTCTGAAACCTTGGCA	50.0	45.0	58.0	58.0	1162 bp	0.5	59.4
ycf1_p5	TTACAGAATTGGGAGCGCTC	CGAAACCGACGTTCGTTTTT	50.0	45.0	58.0	57.9	1012 bp	0.5	59.2