Hotspot

ndhH

SSC · 129771-130952 · 1182 bp

Back to Piptadenia

Pi 0.493

MarkerSeek score 79.10

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1182
variable_sites: 461
pi: 0.493
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	TCAGGTCCAATACGCTGTTG	GAAAACCCGGGATCGATTGA	50.0	50.0	57.9	58.0	1705 bp	0.5	59.0
ndhH_p2	TCAGGTCCAATACGCTGTTG	AGAAAACCCGGGATCGATTG	50.0	50.0	57.9	58.0	1706 bp	0.5	58.9
ndhH_p3	TCAGGTCCAATACGCTGTTG	AAGAAGAAAACCCGGGATCG	50.0	50.0	57.9	57.9	1710 bp	0.5	58.4
ndhH_p4	TCAGGTCCAATACGCTGTTG	CAGTTGGATATTCGGGAGCC	50.0	55.0	57.9	58.1	1513 bp	0.5	58.3
ndhH_p5	GAAGAACTCCCAAAGGACCG	GAAAACCCGGGATCGATTGA	55.0	50.0	58.2	58.0	1731 bp	0.5	58.2