Hotspot

ndhF

SSC · 117651-119882 · 2232 bp

Back to Piptadenia

Pi 0.458

MarkerSeek score 76.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2232
variable_sites: 778
pi: 0.458
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGTTTCGATGCAACAACCAA	TCCCCACTCTCTGGATCTAA	40.0	50.0	56.8	56.4	2885 bp	0.5	37.7
ndhF_p2	TGTTTCGATGCAACAACCAA	TCCCCACTCTCTGGATCTAAA	40.0	47.6	56.8	57.2	2885 bp	0.5	35.9
ndhF_p3	ATGTTTCGATGCAACAACCA	TCCCCACTCTCTGGATCTAA	40.0	50.0	56.5	56.4	2886 bp	0.5	35.9
ndhF_p4	TGTTTCGATGCAACAACCAA	ATCCCCACTCTCTGGATCTA	40.0	50.0	56.8	56.2	2886 bp	0.5	35.8
ndhF_p5	TGTTTCGATGCAACAACCAA	TATCCCCACTCTCTGGATCT	40.0	50.0	56.8	56.2	2887 bp	0.5	35.8