Hotspot

ndhD

SSC · 123211-124707 · 1497 bp

Back to Piptadenia

Pi 0.474

MarkerSeek score 81.80

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1497
variable_sites: 628
pi: 0.474
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	AAACACCAAACGTATCCGGT	TGATCGTGTTTGCGAAAACG	45.0	45.0	57.7	58.0	2212 bp	0.5	57.8
ndhD_p2	AAACACCAAACGTATCCGGT	GATCGTGTTTGCGAAAACGT	45.0	45.0	57.7	58.0	2211 bp	0.5	57.8
ndhD_p3	AAACACCAAACGTATCCGGT	ACGTATGATCGTGTTTGCGA	45.0	45.0	57.7	58.0	2217 bp	0.5	57.8
ndhD_p4	AAACACCAAACGTATCCGGT	TCTGCTCCAAGAACAGAGGA	45.0	50.0	57.7	58.0	1910 bp	0.5	57.8
ndhD_p5	AAACACCAAACGTATCCGGT	AAACAACTCGAAGCATGGGT	45.0	45.0	57.7	58.0	1816 bp	0.5	57.8