Hotspot

ccsA

SSC · 121632-122603 · 972 bp

Back to Piptadenia

Pi 0.435

MarkerSeek score 80.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 350
pi: 0.435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	GGTGTTTGTGAGAACCCCTT	50.0	50.0	58.0	57.9	1585 bp	0.5	59.4
ccsA_p2	TGAAATCGGTAGACACGCTG	GGTGTTTGTGAGAACCCCTT	50.0	50.0	58.0	57.9	1584 bp	0.5	59.4
ccsA_p3	GTGAAATCGGTAGACACGCT	TGGGCCCCGAGAGTTATTTA	50.0	50.0	58.0	58.1	1866 bp	0.5	59.0
ccsA_p4	TGAAATCGGTAGACACGCTG	TGGGCCCCGAGAGTTATTTA	50.0	50.0	58.0	58.1	1865 bp	0.5	59.0
ccsA_p5	GTGAAATCGGTAGACACGCT	CAGTTGACAAGGTCGAAGCT	50.0	50.0	58.0	58.1	1780 bp	0.5	58.9