Hotspot

rps15-ycf1

SSC · 131329-131735 · 407 bp

Back to Piptadenia

Pi 0.452

MarkerSeek score 78.40

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 407
variable_sites: 165
pi: 0.452
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 78.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCAATCGATCCCGGGTTTTC	GCAGCCGTTTCAGTATGGTA	50.0	50.0	58.0	58.0	531 bp	0.5	59.6
rps15-ycf1_p2	CAATCGATCCCGGGTTTTCT	GCAGCCGTTTCAGTATGGTA	50.0	50.0	58.0	58.0	530 bp	0.5	59.5
rps15-ycf1_p3	TCCTAACTGTTCCGGTCCAT	GCAGCCGTTTCAGTATGGTA	50.0	50.0	58.1	58.0	1109 bp	0.5	59.4
rps15-ycf1_p4	TGCATTGATGGATGGTGAGG	GCAGCCGTTTCAGTATGGTA	50.0	50.0	57.9	58.0	907 bp	0.5	59.4
rps15-ycf1_p5	CTTGTAGCTGGGGCATTCAT	GCAGCCGTTTCAGTATGGTA	50.0	50.0	57.9	58.0	859 bp	0.5	59.3