Hotspot

psaC-ndhE

SSC · 125080-125339 · 260 bp

Back to Piptadenia

Pi 0.48

MarkerSeek score 77.00

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 260
variable_sites: 119
pi: 0.48
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	TCCTCTGTTCTTGGAGCAGA	ACCGGCTATTGTTTCGTCAA	50.0	45.0	58.0	57.8	476 bp	0.5	58.5
psaC-ndhE_p2	ACCCATGCTTCGAGTTGTTT	ACCGGCTATTGTTTCGTCAA	45.0	45.0	58.0	57.8	570 bp	0.5	58.5
psaC-ndhE_p3	GCTTCGAGTTGTTTCATGCC	ACCGGCTATTGTTTCGTCAA	50.0	45.0	58.0	57.8	564 bp	0.5	58.4
psaC-ndhE_p4	CCCGTCAGTAAAATAGGGCC	ACCGGCTATTGTTTCGTCAA	55.0	45.0	58.0	57.8	989 bp	0.5	58.3
psaC-ndhE_p5	TCCTCTGTTCTTGGAGCAGA	AGCTGCTTTGATAGGTGCAA	50.0	45.0	58.0	57.8	985 bp	0.5	58.2