Hotspot

rpl32-ndhF

SSC · 129744-130670 · 927 bp

Back to Piper

Pi 0.0407
MarkerSeek score 84.40
Primer available yes
Label rpl32-ndhF

Feature Metadata

label_name
rpl32-ndhF
feature_type
igs
region
SSC
length_bp
927
variable_sites
85
pi
0.0407
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-ndhF_p1 GTACGTTTCTTTGGAACCGC ACAGTAACCGGGAGTGGAAT 50.0 50.0 57.7 58.3 1008-1296 bp 1.0 83.4
rpl32-ndhF_p2 GTACGTTTCTTTGGAACCGC ACCGGGAGTGGAATAAAAGG 50.0 50.0 57.7 57.2 1002-1290 bp 1.0 79.8
rpl32-ndhF_p3 GTACGTTTCTTTGGAACCGC CCGGGAGTGGAATAAAAGGT 50.0 50.0 57.7 57.2 1001-1289 bp 1.0 79.8
rpl32-ndhF_p4 TACGTTTCTTTGGAACCGCC ACAGTAACCGGGAGTGGAAT 50.0 50.0 58.8 58.3 1007-1295 bp 1.0 79.6
rpl32-ndhF_p5 GTACGTTTCTTTGGAACCGC CACGCATATGGGTATGTACGT 50.0 47.6 57.7 58.0 975-1263 bp 1.0 78.2