Hotspot

trnL-UAG-rpl32

SSC · 128546-129578 · 1033 bp

Back to Piper

Pi 0.0306
MarkerSeek score 66.10
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
1033
variable_sites
70
pi
0.0306
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.75
markerseek_score
66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 TTTTATTAGGGGGATGCCGC AGACGGCGCAATTTAGTCAT 50.0 45.0 58.0 58.0 1807-1809 bp 0.285714 51.3
trnL-UAG-rpl32_p2 AGAAACCCCACGAAAGGAAC AGACGGCGCAATTTAGTCAT 50.0 45.0 58.0 58.0 2184-2186 bp 0.285714 51.3
trnL-UAG-rpl32_p3 AGCACCTGTAGCCGTAATTG AGACGGCGCAATTTAGTCAT 50.0 45.0 58.0 58.0 2255-2257 bp 0.285714 51.3
trnL-UAG-rpl32_p4 CCTGTAGCCGTAATTGTGCT AGACGGCGCAATTTAGTCAT 50.0 45.0 58.0 58.0 2251-2253 bp 0.285714 51.3
trnL-UAG-rpl32_p5 TTTTATTAGGGGGATGCCGC AAACGGGGATATTGGGAAGC 50.0 50.0 58.0 57.9 1229-1230 bp 0.285714 51.0