Hotspot

ccsA-trnL-UAG

SSC · 128322-128465 · 144 bp

Back to Piper

Pi 0.0509
MarkerSeek score 73.30
Primer available yes
Label ccsA-trnL-UAG

Feature Metadata

label_name
ccsA-trnL-UAG
feature_type
igs
region
SSC
length_bp
144
variable_sites
17
pi
0.0509
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-trnL-UAG_p1 AGAAACCCCACGAAAGGAAC TAGACACGCTGCTCTTAGGA 50.0 50.0 58.0 57.9 452-463 bp 0.857143 78.2
ccsA-trnL-UAG_p2 AGAAACCCCACGAAAGGAAC AAATTGGTAGACACGCTGCT 50.0 45.0 58.0 57.8 459-470 bp 0.857143 77.6
ccsA-trnL-UAG_p3 AGCACCTGTAGCCGTAATTG TAGACACGCTGCTCTTAGGA 50.0 50.0 58.0 57.9 523 bp 0.285714 50.5
ccsA-trnL-UAG_p4 CCTGTAGCCGTAATTGTGCT TAGACACGCTGCTCTTAGGA 50.0 50.0 58.0 57.9 519 bp 0.285714 50.5
ccsA-trnL-UAG_p5 CCTGTAGCCGTAATTGTGCT AAATTGGTAGACACGCTGCT 50.0 45.0 58.0 57.8 526 bp 0.285714 50.1