Hotspot

ndhD-ccsA

SSC · 127035-127355 · 321 bp

Back to Piper

Pi 0.0626
MarkerSeek score 71.60
Primer available yes
Label ndhD-ccsA

Feature Metadata

label_name
ndhD-ccsA
feature_type
igs
region
SSC
length_bp
321
variable_sites
41
pi
0.0626
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.94
markerseek_score
71.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD-ccsA_p1 AAATGGGAGCATATGGGCTG TTCGGCAATTGTAGCTTCGA 50.0 45.0 58.0 57.9 1132-1180 bp 0.857143 81.2
ndhD-ccsA_p2 CTGCCAGGAATGAGTGGTTT GTTCCTTTCGTGGGGTTTCT 50.0 50.0 58.1 58.0 1364-1412 bp 0.857143 81.0
ndhD-ccsA_p3 AAATGGGAGCATATGGGCTG GTTCCTTTCGTGGGGTTTCT 50.0 50.0 58.0 58.0 1762-1810 bp 0.857143 80.4
ndhD-ccsA_p4 AAATGGGAGCATATGGGCTG AGCACAATTACGGCTACAGG 50.0 50.0 58.0 58.0 1727-1743 bp 0.285714 51.2
ndhD-ccsA_p5 AAATGGGAGCATATGGGCTG CAATTACGGCTACAGGTGCT 50.0 50.0 58.0 58.0 1723-1739 bp 0.285714 51.2