Hotspot

trnN-GUU-rps15

SSC · 112826-119052 · 6227 bp

Back to Piper

Pi 0.0227
MarkerSeek score 57.20
Primer available yes
Label trnN-GUU-rps15

Feature Metadata

label_name
trnN-GUU-rps15
feature_type
igs
region
SSC
length_bp
6227
variable_sites
316
pi
0.0227
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-rps15_p1 AGTAGTACCGACGACTATGCA CTTGTCCCTGCCCTTCTTTT 47.6 50.0 58.1 58.0 1351 bp 0.285714 42.9
trnN-GUU-rps15_p2 AGTAGTACCGACGACTATGCA TGTCCCTGCCCTTCTTTTTC 47.6 50.0 58.1 58.0 1349 bp 0.285714 42.9
trnN-GUU-rps15_p3 AGTAGTACCGACGACTATGCA GGAAAGAACCAAGGCTCGAT 47.6 50.0 58.1 57.9 1145 bp 0.285714 42.1
trnN-GUU-rps15_p4 AGTAGTACCGACGACTATGCA GTCTTGACTAAAGGGCCTGG 47.6 55.0 58.1 57.9 972 bp 0.285714 42.1
trnN-GUU-rps15_p5 AGTAGTACCGACGACTATGCA GAACCAAGGCTCGATACTCG 47.6 55.0 58.1 58.2 1140 bp 0.285714 41.7