Pi
0.0277
MarkerSeek score
71.10
Primer available
yes
Label
petA-psbJ
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| petA-psbJ_p1 | TACGGGTCCAAGGTTTGTTG | ATTCTGGATTGGGCTCATCC | 50.0 | 50.0 | 58.0 | 57.3 | 993-1005 bp | 1.0 | 80.1 |
| petA-psbJ_p2 | GCATCTGTTACTCTGGCACA | ATTCTGGATTGGGCTCATCC | 50.0 | 50.0 | 57.9 | 57.3 | 964-976 bp | 1.0 | 79.7 |
| petA-psbJ_p3 | TTGGCATCTGTTACTCTGGC | ATTCTGGATTGGGCTCATCC | 50.0 | 50.0 | 57.9 | 57.3 | 967-979 bp | 1.0 | 79.5 |
| petA-psbJ_p4 | CCATTACGGGTCCAAGGTTT | ATTCTGGATTGGGCTCATCC | 50.0 | 50.0 | 57.8 | 57.3 | 997-1009 bp | 1.0 | 78.6 |
| petA-psbJ_p5 | GATCCATTACGGGTCCAAGG | ATTCTGGATTGGGCTCATCC | 55.0 | 50.0 | 57.7 | 57.3 | 1000-1012 bp | 1.0 | 78.2 |