Hotspot

trnY-GUA-trnE-UUC

LSC · 32020-32078 · 59 bp

Back to Piper

Pi 0.0581
MarkerSeek score 73.00
Primer available yes
Label trnY-GUA-trnE-UUC

Feature Metadata

label_name
trnY-GUA-trnE-UUC
feature_type
igs
region
LSC
length_bp
59
variable_sites
8
pi
0.0581
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnE-UUC_p1 TGGGAATGGCGAATTAGTGG CTCTCTTTCAAGGAGGCAGC 50.0 55.0 57.9 58.3 205 bp 1.0 80.6
trnY-GUA-trnE-UUC_p2 TGGGAATGGCGAATTAGTGG CCCCTATCGTCTAGTGGTCA 50.0 55.0 57.9 57.6 231 bp 1.0 79.5
trnY-GUA-trnE-UUC_p3 TGGGAATGGCGAATTAGTGG ATGCCCCTATCGTCTAGTGG 50.0 55.0 57.9 58.4 234 bp 1.0 79.4
trnY-GUA-trnE-UUC_p4 TGGGAATGGCGAATTAGTGG TCGTCTAGTGGTCAGGACAT 50.0 50.0 57.9 57.5 225 bp 1.0 78.4
trnY-GUA-trnE-UUC_p5 TGGGAATGGCGAATTAGTGG ATCGTCTAGTGGTCAGGACA 50.0 50.0 57.9 57.5 226 bp 1.0 78.3