Hotspot

psbM-trnD-GUC

LSC · 30517-31537 · 1021 bp

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Pi 0.0315
MarkerSeek score 76.80
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1021
variable_sites
71
pi
0.0315
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAATAGCACAGTAGCA AGTTCAATCGGTCAGAGCAC 38.1 50.0 54.7 57.9 1134-1178 bp 1.0 52.5
psbM-trnD-GUC_p2 AGAATGAATAGCACAGTAGCAA AGTTCAATCGGTCAGAGCAC 36.4 50.0 55.5 57.9 1134-1178 bp 1.0 50.9
psbM-trnD-GUC_p3 ACTAGAATGAATAGCACAGTAGCA AGTTCAATCGGTCAGAGCAC 37.5 50.0 57.5 57.9 1137-1181 bp 1.0 50.8
psbM-trnD-GUC_p4 AGGAACTAGAATGAATAGCACAGT AGTTCAATCGGTCAGAGCAC 37.5 50.0 57.4 57.9 1141-1185 bp 1.0 50.1
psbM-trnD-GUC_p5 AGAATGAATAGCACAGTAGCA CCGGGATTCTGGGATTGTAG 38.1 55.0 54.7 57.4 1152-1196 bp 1.0 48.7