primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	CAATCCACTGCCTTGATCCA	AACCGTGCAAACCTTGGTAT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.143575	102	293	327-330	1.000000	86.9
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	AATCCACTGCCTTGATCCAC	AACCGTGCAAACCTTGGTAT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.145083	102	293	326-329	1.000000	86.8
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	ACAATCCACTGCCTTGATCC	AACCGTGCAAACCTTGGTAT	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.145083	102	293	328-331	1.000000	86.8
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	CTTGATCCACTTGGCCACAT	AACCGTGCAAACCTTGGTAT	20	20	50.0	45.0	58.2	58.0	3.9	0.0	0.166925	102	293	316-319	1.000000	86.7
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	CAATCCACTGCCTTGATCCA	GCGTAATGCACACAACTTCC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.169535	102	293	292-295	1.000000	86.6
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.2	58.2	20.3	1.3	0.358736	4294	5289	1139-1182	1.000000	85.0
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.2	58.2	20.3	1.3	0.358736	4294	5289	1138-1181	1.000000	85.0
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	TACCGTTGAGTTAGCAACCC	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	57.5	58.2	0.0	1.3	0.651073	4294	5289	1125-1168	1.000000	82.8
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AGTCGCACTTAAAAGCCGAG	GGTGCTCAACCTACAGGAAC	20	20	50.0	55.0	58.6	58.2	6.4	1.3	0.760853	4294	5289	1150-1193	1.000000	81.7
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCGCACTTAAAAGCCGAGTA	GGTGCTCAACCTACAGGAAC	20	20	45.0	55.0	57.3	58.2	3.0	1.3	0.919006	4294	5289	1148-1191	1.000000	80.5
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	20	20	45.0	55.0	58.1	58.1	8.1	16.9	0.215425	6391	7344	1023-1318	1.000000	85.3
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	TGATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	20	20	50.0	55.0	58.2	58.1	0.0	16.9	0.280333	6391	7344	1034-1329	1.000000	84.7
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	20	20	45.0	55.0	58.1	57.8	8.1	16.9	0.350677	6391	7344	1027-1322	1.000000	84.2
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	TGATGTCCTTCAAGTCGCAC	CTCGGAGGTTCGAATCCTTC	20	20	50.0	55.0	58.2	57.8	0.0	16.9	0.415585	6391	7344	1038-1333	1.000000	83.6
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	AAGTCGCACGTTGCTTTCTA	ACATATAAATGGGGCGTGGC	20	20	45.0	50.0	58.1	58.4	8.1	0.0	0.523165	6391	7344	1080-1375	1.000000	82.5
trnQ-UUG-psbK_p1	trnQ-UUG-psbK	1	GAAGGATTCGAACCTCCGAG	ACTCGAATGAAGGGCAGAAC	20	20	55.0	50.0	57.8	57.9	16.4	0.0	0.311957	7418	7741	439-439	1.000000	82.2
trnQ-UUG-psbK_p2	trnQ-UUG-psbK	2	GAAGGATTCGAACCTCCGAG	AAGCTACTCGAATGAAGGGC	20	20	55.0	50.0	57.8	57.7	16.4	0.0	0.528882	7418	7741	444-444	1.000000	80.5
trnQ-UUG-psbK_p3	trnQ-UUG-psbK	3	AGGATTCGAACCTCCGAGTA	ACTCGAATGAAGGGCAGAAC	20	20	50.0	50.0	57.6	57.9	11.3	0.0	0.532558	7418	7741	437-437	1.000000	80.5
trnQ-UUG-psbK_p4	trnQ-UUG-psbK	4	AAGGATTCGAACCTCCGAGT	ACTCGAATGAAGGGCAGAAC	20	20	50.0	50.0	58.4	57.9	16.4	0.0	0.542372	7418	7741	438-438	1.000000	80.4
trnQ-UUG-psbK_p5	trnQ-UUG-psbK	5	GAGTAACGGGACCAAAACCC	ACTCGAATGAAGGGCAGAAC	20	20	55.0	50.0	58.5	57.9	0.0	0.0	0.573570	7418	7741	422-422	1.000000	80.3
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	AGAATGAATAGCACAGTAGCA	AGTTCAATCGGTCAGAGCAC	21	20	38.1	50.0	54.7	57.9	0.0	0.0	4.347921	30517	31537	1134-1178	1.000000	52.5
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AGAATGAATAGCACAGTAGCAA	AGTTCAATCGGTCAGAGCAC	22	20	36.4	50.0	55.5	57.9	0.0	0.0	4.550235	30517	31537	1134-1178	1.000000	50.9
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	ACTAGAATGAATAGCACAGTAGCA	AGTTCAATCGGTCAGAGCAC	24	20	37.5	50.0	57.5	57.9	0.0	0.0	4.557288	30517	31537	1137-1181	1.000000	50.8
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGGAACTAGAATGAATAGCACAGT	AGTTCAATCGGTCAGAGCAC	24	20	37.5	50.0	57.4	57.9	0.0	0.0	4.638499	30517	31537	1141-1185	1.000000	50.1
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGAATGAATAGCACAGTAGCA	CCGGGATTCTGGGATTGTAG	21	20	38.1	55.0	54.7	57.4	0.0	0.0	4.812697	30517	31537	1152-1196	1.000000	48.7
trnY-GUA-trnE-UUC_p1	trnY-GUA-trnE-UUC	1	TGGGAATGGCGAATTAGTGG	CTCTCTTTCAAGGAGGCAGC	20	20	50.0	55.0	57.9	58.3	0.0	0.0	0.314723	32020	32078	205-205	1.000000	80.6
trnY-GUA-trnE-UUC_p2	trnY-GUA-trnE-UUC	2	TGGGAATGGCGAATTAGTGG	CCCCTATCGTCTAGTGGTCA	20	20	50.0	55.0	57.9	57.6	0.0	0.0	0.411316	32020	32078	231-231	1.000000	79.5
trnY-GUA-trnE-UUC_p3	trnY-GUA-trnE-UUC	3	TGGGAATGGCGAATTAGTGG	ATGCCCCTATCGTCTAGTGG	20	20	50.0	55.0	57.9	58.4	0.0	0.0	0.417561	32020	32078	234-234	1.000000	79.4
trnY-GUA-trnE-UUC_p4	trnY-GUA-trnE-UUC	4	TGGGAATGGCGAATTAGTGG	TCGTCTAGTGGTCAGGACAT	20	20	50.0	50.0	57.9	57.5	0.0	1.4	0.560501	32020	32078	225-225	1.000000	78.4
trnY-GUA-trnE-UUC_p5	trnY-GUA-trnE-UUC	5	TGGGAATGGCGAATTAGTGG	ATCGTCTAGTGGTCAGGACA	20	20	50.0	50.0	57.9	57.5	0.0	1.4	0.560501	32020	32078	226-226	1.000000	78.3
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	AGTGGGTATCCTTAATTCTCTCA	AATCGAACCCGCATCTTCTC	23	20	39.1	50.0	56.2	58.1	0.0	0.0	4.887854	37804	38091	377-379	1.000000	47.9
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	AGTGGGTATCCTTAATTCTCTCA	GAATCGAACCCGCATCTTCT	23	20	39.1	50.0	56.2	58.1	0.0	0.0	4.887854	37804	38091	378-380	1.000000	47.8
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	GTGGGTATCCTTAATTCTCTCA	AATCGAACCCGCATCTTCTC	22	20	40.9	50.0	54.7	58.1	0.0	0.0	5.360994	37804	38091	376-378	1.000000	47.0
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	TGGATTAGTTTTTCTAGTGGGT	AATCGAACCCGCATCTTCTC	22	20	36.4	50.0	54.7	58.1	0.0	0.0	5.347973	37804	38091	392-394	1.000000	46.7
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	TGGATTAGTTTTTCTAGTGGGT	GAATCGAACCCGCATCTTCT	22	20	36.4	50.0	54.7	58.1	0.0	0.0	5.347973	37804	38091	393-395	1.000000	46.7
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	48150	48955	856-906	1.000000	83.6
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	48150	48955	864-914	1.000000	83.0
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	ACAAATAGAACAAGCCGGCT	ACCAATTTCGCCATATCCCC	20	20	45.0	50.0	57.8	58.0	27.1	0.0	0.204996	48150	48955	911-961	1.000000	82.8
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	48150	48955	888-938	1.000000	82.6
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	ACAAATAGAACAAGCCGGCT	TAGCGTCTACCAATTTCGCC	20	20	45.0	50.0	57.8	58.1	27.1	0.0	0.269805	48150	48955	919-969	1.000000	82.2
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TGCCAAAATAGGAATAACACTTGA	AGAAGGTCTACGGTTCGAGT	24	20	33.3	50.0	56.8	57.8	0.0	0.0	5.352136	52385	53729	1449-1594	1.000000	46.1
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TGCCAAAATAGGAATAACACTTGA	GAAGGTCTACGGTTCGAGTC	24	20	33.3	55.0	56.8	57.5	0.0	0.0	5.695908	52385	53729	1448-1593	1.000000	46.1
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TGCCAAAATAGGAATAACACTTGA	GAGCCCAATGCAAAGCAAAA	24	20	33.3	45.0	56.8	58.1	0.0	0.0	5.273262	52385	53729	1537-1685	1.000000	45.8
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TGCCAAAATAGGAATAACACTTGA	AGCCCAATGCAAAGCAAAAA	24	20	33.3	40.0	56.8	57.7	0.0	0.0	5.499977	52385	53729	1536-1684	1.000000	45.8
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	TGCCAAAATAGGAATAACACTTG	GAGCCCAATGCAAAGCAAAA	23	20	34.8	45.0	55.5	58.1	0.0	0.0	5.653014	52385	53729	1537-1685	1.000000	45.8
petA-psbJ_p1	petA-psbJ	1	TACGGGTCCAAGGTTTGTTG	ATTCTGGATTGGGCTCATCC	20	20	50.0	50.0	58.0	57.3	0.0	7.8	0.701804	66006	66865	993-1005	1.000000	80.1
petA-psbJ_p2	petA-psbJ	2	GCATCTGTTACTCTGGCACA	ATTCTGGATTGGGCTCATCC	20	20	50.0	50.0	57.9	57.3	0.0	7.8	0.771037	66006	66865	964-976	1.000000	79.7
petA-psbJ_p3	petA-psbJ	3	TTGGCATCTGTTACTCTGGC	ATTCTGGATTGGGCTCATCC	20	20	50.0	50.0	57.9	57.3	0.0	7.8	0.785756	66006	66865	967-979	1.000000	79.5
petA-psbJ_p4	petA-psbJ	4	CCATTACGGGTCCAAGGTTT	ATTCTGGATTGGGCTCATCC	20	20	50.0	50.0	57.8	57.3	0.0	7.8	0.881586	66006	66865	997-1009	1.000000	78.6
petA-psbJ_p5	petA-psbJ	5	GATCCATTACGGGTCCAAGG	ATTCTGGATTGGGCTCATCC	20	20	55.0	50.0	57.7	57.3	0.0	7.8	0.928013	66006	66865	1000-1012	1.000000	78.2
trnN-GUU-rps15_p1	trnN-GUU-rps15	1	AGTAGTACCGACGACTATGCA	CTTGTCCCTGCCCTTCTTTT	21	20	47.6	50.0	58.1	58.0	0.0	0.0	1.090876	117114	117233	1351-1351	0.285714	42.9
trnN-GUU-rps15_p2	trnN-GUU-rps15	2	AGTAGTACCGACGACTATGCA	TGTCCCTGCCCTTCTTTTTC	21	20	47.6	50.0	58.1	58.0	0.0	0.0	1.094424	117114	117233	1349-1349	0.285714	42.9
trnN-GUU-rps15_p3	trnN-GUU-rps15	3	AGTAGTACCGACGACTATGCA	GGAAAGAACCAAGGCTCGAT	21	20	47.6	50.0	58.1	57.9	0.0	0.0	1.205143	117114	117233	1145-1145	0.285714	42.1
trnN-GUU-rps15_p4	trnN-GUU-rps15	4	AGTAGTACCGACGACTATGCA	GTCTTGACTAAAGGGCCTGG	21	20	47.6	55.0	58.1	57.9	0.0	6.9	1.205143	117114	117233	972-972	0.285714	42.1
trnN-GUU-rps15_p5	trnN-GUU-rps15	5	AGTAGTACCGACGACTATGCA	GAACCAAGGCTCGATACTCG	21	20	47.6	55.0	58.1	58.2	0.0	0.0	1.248351	117114	117233	1140-1140	0.285714	41.7
ndhG-ndhE_p1	ndhG-ndhE	1	TGGGGATGGGGTTACTTCAT	ACATCCGTCCCAAGGTATCA	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.131472	124334	124564	1190-1213	1.000000	83.3
ndhG-ndhE_p2	ndhG-ndhE	2	TGGGGATGGGGTTACTTCAT	ATCATTTCCAACACGTCCGT	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.184513	124334	124564	1174-1197	1.000000	82.9
ndhG-ndhE_p3	ndhG-ndhE	3	TGGGGATGGGGTTACTTCAT	CGAGTTTTTGGGCGTTTTCA	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.201896	124334	124564	1079-1080	0.285714	49.9
ndhG-ndhE_p4	ndhG-ndhE	4	CTGCATGAATTCGCATTGCA	ACATCCGTCCCAAGGTATCA	20	20	45.0	50.0	58.1	58.1	18.2	0.0	0.209918	124334	124564	1617-1618	0.285714	49.8
ndhG-ndhE_p5	ndhG-ndhE	5	CTGCATGAATTCGCATTGCA	ATCATTTCCAACACGTCCGT	20	20	45.0	45.0	58.1	57.8	18.2	0.0	0.262960	124334	124564	1601-1602	0.285714	49.4
ndhD-ccsA_p1	ndhD-ccsA	1	AAATGGGAGCATATGGGCTG	TTCGGCAATTGTAGCTTCGA	20	20	50.0	45.0	58.0	57.9	4.6	7.4	0.092871	127035	127355	1132-1180	0.857143	81.2
ndhD-ccsA_p2	ndhD-ccsA	2	CTGCCAGGAATGAGTGGTTT	GTTCCTTTCGTGGGGTTTCT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.107803	127035	127355	1364-1412	0.857143	81.0
ndhD-ccsA_p3	ndhD-ccsA	3	AAATGGGAGCATATGGGCTG	GTTCCTTTCGTGGGGTTTCT	20	20	50.0	50.0	58.0	58.0	4.6	0.0	0.028314	127035	127355	1762-1810	0.857143	80.4
ndhD-ccsA_p4	ndhD-ccsA	4	AAATGGGAGCATATGGGCTG	AGCACAATTACGGCTACAGG	20	20	50.0	50.0	58.0	58.0	4.6	0.0	0.029546	127035	127355	1727-1743	0.285714	51.2
ndhD-ccsA_p5	ndhD-ccsA	5	AAATGGGAGCATATGGGCTG	CAATTACGGCTACAGGTGCT	20	20	50.0	50.0	58.0	58.0	4.6	0.0	0.029546	127035	127355	1723-1739	0.285714	51.2
ccsA-trnL-UAG_p1	ccsA-trnL-UAG	1	AGAAACCCCACGAAAGGAAC	TAGACACGCTGCTCTTAGGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.147221	128322	128465	452-463	0.857143	78.2
ccsA-trnL-UAG_p2	ccsA-trnL-UAG	2	AGAAACCCCACGAAAGGAAC	AAATTGGTAGACACGCTGCT	20	20	50.0	45.0	58.0	57.8	0.0	0.0	0.207948	128322	128465	459-470	0.857143	77.6
ccsA-trnL-UAG_p3	ccsA-trnL-UAG	3	AGCACCTGTAGCCGTAATTG	TAGACACGCTGCTCTTAGGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.148453	128322	128465	523-523	0.285714	50.5
ccsA-trnL-UAG_p4	ccsA-trnL-UAG	4	CCTGTAGCCGTAATTGTGCT	TAGACACGCTGCTCTTAGGA	20	20	50.0	50.0	58.0	57.9	4.1	0.0	0.148453	128322	128465	519-519	0.285714	50.5
ccsA-trnL-UAG_p5	ccsA-trnL-UAG	5	CCTGTAGCCGTAATTGTGCT	AAATTGGTAGACACGCTGCT	20	20	50.0	45.0	58.0	57.8	4.1	0.0	0.209180	128322	128465	526-526	0.285714	50.1
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	TTTTATTAGGGGGATGCCGC	AGACGGCGCAATTTAGTCAT	20	20	50.0	45.0	58.0	58.0	12.8	10.8	0.034469	128546	129578	1807-1809	0.285714	51.3
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	AGAAACCCCACGAAAGGAAC	AGACGGCGCAATTTAGTCAT	20	20	50.0	45.0	58.0	58.0	0.0	10.8	0.047119	128546	129578	2184-2186	0.285714	51.3
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	AGCACCTGTAGCCGTAATTG	AGACGGCGCAATTTAGTCAT	20	20	50.0	45.0	58.0	58.0	0.0	10.8	0.048351	128546	129578	2255-2257	0.285714	51.3
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	CCTGTAGCCGTAATTGTGCT	AGACGGCGCAATTTAGTCAT	20	20	50.0	45.0	58.0	58.0	4.1	10.8	0.048351	128546	129578	2251-2253	0.285714	51.3
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	TTTTATTAGGGGGATGCCGC	AAACGGGGATATTGGGAAGC	20	20	50.0	50.0	58.0	57.9	12.8	0.0	0.077659	128546	129578	1229-1230	0.285714	51.0
rpl32-ndhF_p1	rpl32-ndhF	1	GTACGTTTCTTTGGAACCGC	ACAGTAACCGGGAGTGGAAT	20	20	50.0	50.0	57.7	58.3	0.0	6.3	0.652305	129744	130670	1008-1296	1.000000	83.4
rpl32-ndhF_p2	rpl32-ndhF	2	GTACGTTTCTTTGGAACCGC	ACCGGGAGTGGAATAAAAGG	20	20	50.0	50.0	57.7	57.2	0.0	6.3	1.106328	129744	130670	1002-1290	1.000000	79.8
rpl32-ndhF_p3	rpl32-ndhF	3	GTACGTTTCTTTGGAACCGC	CCGGGAGTGGAATAAAAGGT	20	20	50.0	50.0	57.7	57.2	0.0	0.0	1.106328	129744	130670	1001-1289	1.000000	79.8
rpl32-ndhF_p4	rpl32-ndhF	4	TACGTTTCTTTGGAACCGCC	ACAGTAACCGGGAGTGGAAT	20	20	50.0	50.0	58.8	58.3	0.0	6.3	1.118520	129744	130670	1007-1295	1.000000	79.6
rpl32-ndhF_p5	rpl32-ndhF	5	GTACGTTTCTTTGGAACCGC	CACGCATATGGGTATGTACGT	20	21	50.0	47.6	57.7	58.0	0.0	23.4	1.326521	129744	130670	975-1263	1.000000	78.2