Hotspot

ycf2

Genome · 112634-118777 · 6144 bp

Back to Pinus

Pi 0.000732
MarkerSeek score 53.00
Primer available yes
Label ycf2

Feature Metadata

label_name
ycf2
feature_type
gene
region
Genome
length_bp
6144
variable_sites
9
pi
0.000732
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2_p1 ATGGCTGCATACGTTCCAAT CGCCGAGAATTTTTCGCAAA 45.0 45.0 57.9 58.3 256 bp 1.0 77.6
ycf2_p2 TGGCTGCATACGTTCCAATT CGCCGAGAATTTTTCGCAAA 45.0 45.0 58.2 58.3 255 bp 1.0 76.7
ycf2_p3 GCTGCATACGTTCCAATTGG CGCCGAGAATTTTTCGCAAA 50.0 45.0 57.8 58.3 253 bp 1.0 76.5
ycf2_p4 GGCTGCATACGTTCCAATTG CGCCGAGAATTTTTCGCAAA 50.0 45.0 57.8 58.3 254 bp 1.0 76.5
ycf2_p5 ATTTTCGGTCAACCAACGGT CGCCGAGAATTTTTCGCAAA 45.0 45.0 58.3 58.3 297 bp 1.0 75.4