Hotspot

ycf1

Genome · 95561-100831 · 5271 bp

Back to Pinus

Pi 0.000601
MarkerSeek score 53.20
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
Genome
length_bp
5271
variable_sites
6
pi
0.000601
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 ATGTGCTTCTGATGGTACCG TGGAACGCGTCTTATTCCTG 50.0 50.0 57.7 58.0 292 bp 1.0 77.7
ycf1_p2 ATGTGCTTCTGATGGTACCG GGAACGCGTCTTATTCCTGT 50.0 50.0 57.7 58.0 291 bp 1.0 77.7
ycf1_p3 ATGTGCTTCTGATGGTACCG GTGGAACGCGTCTTATTCCT 50.0 50.0 57.7 58.0 293 bp 1.0 77.7
ycf1_p4 GTGCTTCTGATGGTACCGAA TGGAACGCGTCTTATTCCTG 50.0 50.0 57.6 58.0 290 bp 1.0 77.2
ycf1_p5 GTGCTTCTGATGGTACCGAA GGAACGCGTCTTATTCCTGT 50.0 50.0 57.6 58.0 289 bp 1.0 77.2