Hotspot

rps4

Genome · 69543-70148 · 606 bp

Pi 0.0033

MarkerSeek score 47.40

Primer available yes

Label rps4

Feature Metadata

label_name: rps4
feature_type: gene
region: Genome
length_bp: 606
variable_sites: 4
pi: 0.0033
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4_p1	TCCAGTAGTACTCATACGGGT	AATTTCCAGATGCCCAGCAA	47.6	45.0	57.1	58.1	726-735 bp	1.0	65.4
rps4_p2	TCCAGTAGTACTCATACGGGT	GGGGAAGGGAAAAAGAAGGG	47.6	55.0	57.1	58.1	756-765 bp	1.0	65.4
rps4_p3	TCCAGTAGTACTCATACGGGT	AACCCACCCCTCTCTTTCAT	47.6	50.0	57.1	58.2	704-713 bp	1.0	64.0
rps4_p4	TCCAGTAGTACTCATACGGGT	ACCCACCCCTCTCTTTCATT	47.6	50.0	57.1	58.2	703-712 bp	1.0	64.0
rps4_p5	TCCAGTAGTACTCATACGGGTT	AATTTCCAGATGCCCAGCAA	45.5	45.0	57.8	58.1	726-735 bp	1.0	63.0