Hotspot

trnV-GAC-rps12

Genome · 108342-109723 · 1382 bp

Back to Pinus

Pi 0.000724
MarkerSeek score 59.40
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
Genome
length_bp
1382
variable_sites
2
pi
0.000724
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 GTCAAGGTGACACTCTACCG TGGCAACTTTACGTAAGGCA 55.0 45.0 57.7 57.7 1462-1465 bp 1.0 75.4
trnV-GAC-rps12_p2 GGCTCGAACTGATGACTTCC TGGCAACTTTACGTAAGGCA 55.0 45.0 58.4 57.7 1487-1490 bp 1.0 75.3
trnV-GAC-rps12_p3 GCTCGAACTGATGACTTCCA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.6 57.7 1486-1489 bp 1.0 75.2
trnV-GAC-rps12_p4 CAGGCTCGAACTGATGACTT TGGCAACTTTACGTAAGGCA 50.0 45.0 57.6 57.7 1489-1492 bp 1.0 75.1
trnV-GAC-rps12_p5 TGACACTCTACCGCTGAGTT TGGCAACTTTACGTAAGGCA 50.0 45.0 58.4 57.7 1455-1458 bp 1.0 75.1