Hotspot

psaC-ccsA

Genome · 103786-104943 · 1158 bp

Back to Pinus

Pi 0.00561
MarkerSeek score 53.00
Primer available yes
Label psaC-ccsA

Feature Metadata

label_name
psaC-ccsA
feature_type
igs
region
Genome
length_bp
1158
variable_sites
10
pi
0.00561
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ccsA_p1 GAATCCGCTTGTCCAACAGA GGATCCGTTATTTGGGGGTC 50.0 55.0 58.2 58.0 1310-1311 bp 1.0 78.9
psaC-ccsA_p2 TCCGCTTGTCCAACAGATTT GGATCCGTTATTTGGGGGTC 45.0 55.0 57.7 58.0 1307-1308 bp 1.0 78.3
psaC-ccsA_p3 CAGGTTGTAAGCGGTGTGAA GGATCCGTTATTTGGGGGTC 50.0 55.0 58.4 58.0 1327-1328 bp 1.0 77.1
psaC-ccsA_p4 TGAATCCGCTTGTCCAACAG GGATCCGTTATTTGGGGGTC 50.0 55.0 58.5 58.0 1311-1312 bp 1.0 76.6
psaC-ccsA_p5 AAGACTGCGCAGGTTGTAAG GGATCCGTTATTTGGGGGTC 50.0 55.0 58.5 58.0 1336-1337 bp 1.0 76.5