Hotspot

rps15-psaC

Genome · 101422-103539 · 2118 bp

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Pi 0.000236
MarkerSeek score 57.70
Primer available yes
Label rps15-psaC

Feature Metadata

label_name
rps15-psaC
feature_type
igs
region
Genome
length_bp
2118
variable_sites
1
pi
0.000236
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-psaC_p1 GGTCAATTGGGTATCCGTGG GTACGCATTGGGTACAACCA 55.0 50.0 58.3 58.2 2208-2209 bp 1.0 76.0
rps15-psaC_p2 GTCAATTGGGTATCCGTGGG GTACGCATTGGGTACAACCA 55.0 50.0 58.3 58.2 2207-2208 bp 1.0 76.0
rps15-psaC_p3 TGGTCAATTGGGTATCCGTG GTACGCATTGGGTACAACCA 50.0 50.0 57.6 58.2 2209-2210 bp 1.0 75.3
rps15-psaC_p4 GGGTATCCGTGGGCTAAAAA GTACGCATTGGGTACAACCA 50.0 50.0 57.6 58.2 2200-2201 bp 1.0 75.2
rps15-psaC_p5 ATTGGGTATCCGTGGGCTAA GTACGCATTGGGTACAACCA 50.0 50.0 58.5 58.2 2203-2204 bp 1.0 74.7